Journal: Nutrients

Article Title: A Hypomethylating Ketogenic Diet in Apolipoprotein E-Deficient Mice: A Pilot Study on Vascular Effects and Specific Epigenetic Changes

doi: 10.3390/nu13103576

Figure Lengend Snippet: The effect of the experimental diets (ketogenic diet, KD; low methyl ketogenic diet, LMKD; and control) on the vascular content of specific epigenetic tags at lysine 27 on histone H3 (H2K27me3, tri-methylation; H3K27ac, acetylation) in the aortic arch and brachiocephalic artery (BCA). ( A ) Representative images of brightfield and immunofluorescence quantification of BCA cross sections. Total histone 3 (H3) in green, and histone 3 trimethylation at lysine 27 (H3K27me3) or acetylation (H3K27ac) in magenta. Scale bars = 200 μm. ( B ) Immunohistochemistry results. Data are the mean ± SD, n = 35. Bars bearing different letters were significantly different ( p < 0.05).

Article Snippet: The antibodies used were: rabbit anti-histone H3K27me3 pAb (1:200, Epigentek, Cat.# A-4039), rabbit anti-histone H3K27ac pAb (1:200, Epigentek, Cat.#A-4708), mouse anti-histone H3 mAb 1B1-B2 (1:200, BioLegend, cat.#819404), Alexa Fluor 555 goat anti-rabbit IgG (1:250, Invitrogen, Cat.# A-21428) and Alexa Fluor 488 goat anti-mouse IgG3 (1:250, Invitrogen, Cat.# A-21151).

Techniques: Control, Methylation, Immunofluorescence, Immunohistochemistry

Journal: NPJ Precision Oncology

Article Title: ALDH1A1 promotes PARP inhibitor resistance by enhancing retinoic acid receptor-mediated DNA polymerase θ expression

doi: 10.1038/s41698-023-00411-x

Figure Lengend Snippet: a The conserved base sequence of RARα binding site was identified using JASPAR. b The putative RARE was identified in the promoter region of the POLQ gene. c The ChIP assay with the anti-RARα antibody and normal IgG was conducted to analyze the binding of RARα to the promoter region of the POLQ gene. d , e The ChIP assay with the anti-H3K27ac antibody was conducted to determine the effect of RAR agonist CH55, RA ( d ), and ALDH1A1 inhibitor NCT-505 ( e ) on the histone H3K27 acetylation around the RARE region of the POLQ gene. f – h The ChIP assay with the anti-H3K27me3 antibody was conducted to determine the effect of CH55 ( f ), RAR antagonist AGN 193109 ( g ), and NCT-505 ( h ) on the histone H3K27 methylation around the RARE region of the POLQ gene. Measurements were taken from distinct samples. N = 3, bar: s.d., ** P < 0.01.

Article Snippet: The following antibodies were used for immunoprecipitation: rabbit anti-RARα antibody (C15310155, Diagenode), rabbit anti-H3K27ac antibody (Cat. No. 9649, Cell Signaling Technologies), rabbit anti-H3K27me3 (Cat. No. 39155, Active Motif), and normal rabbit IgG (Cat. No. 2729, Cell Signaling Technologies).

Techniques: Sequencing, Binding Assay, Methylation